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Launch efficacy was expressed as a share of maximal launch, which was determined using saturating concentrations of the non-selective releasing agent tyramine at 10 μM for DAT and NET, and 100 μM for SERT. For in vivo
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As soon as the holin has permeabilized the host mobile membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.
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